Peptide Dose-Response Curves: EC50, Emax and Assay Design
A laboratory guide to designing and interpreting peptide concentration-response experiments without confusing potency, efficacy and system bias.
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The North Specs research library
Cited briefings on peptide science, analytical methods, Canadian research requirements and reproducible laboratory practice.
For qualified research audiences. This library is educational, not medical advice or a protocol for human or veterinary use.
All briefingsRegional ResearchCompound BriefingsAnalytical MethodsExperimental DesignCanada & RegulationMetabolic ResearchStability & HandlingResearch LandscapeMethodologyTissue RepairLongevity ResearchBioregulation & Signalling
Peptide Reference Standards and System Suitability
How reference standards, secondary standards and system-suitability samples support defensible peptide identification and quantitation.
Read briefingHow to Read a Peptide Certificate of Analysis
A field-by-field guide to peptide certificates of analysis, including identity, purity, content, counter-ions, dates, traceability and method limitations.
Read briefingEndotoxin and Bioburden in Peptide Research: Matching Tests to the Model
Endotoxin, bioburden and sterility answer different questions. This guide helps laboratories select controls appropriate to peptide experiments.
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Low-Binding Tubes and Peptide Adsorption: Preventing Invisible Sample Loss
Surface adsorption can distort peptide recovery at low concentration. Learn how material, concentration, time and additives change apparent potency.
Read briefingPeptide Freeze-Thaw Stability: Designing a Defensible Study
How to test peptide freeze-thaw stability using realistic cycles, stability-indicating methods, matched controls and meaningful acceptance criteria.
Read briefingPeptide Solubility, Aggregation and Buffer Selection
A decision framework for selecting peptide solvents and buffers while distinguishing apparent solubility from stable, monomeric recovery.
Read briefingPeptide Lyophilization, Water Content and Storage: A Laboratory Guide
A scientific overview of freeze-drying, residual water, hygroscopicity and the storage records needed to preserve peptide research material.
Read briefingPeptide Counter-Ions: TFA, Acetate and Their Experimental Consequences
Why synthetic peptides carry counter-ions, how TFA and acetate affect mass and assays, and what to document when comparing materials.
Read briefingPeptide Content Versus Purity: Why Net Mass Changes Quantitative Experiments
Purity, gross vial mass and net peptide content are not interchangeable. This guide explains how water, counter-ions and salts affect concentration calculations.
Read briefingLC-MS for Peptide Identity: Molecular Mass, Charge States and Sequence Evidence
How LC-MS supports peptide identity, why charge-state envelopes require deconvolution and when intact mass should be supplemented by sequence-level evidence.
Read briefingHPLC Peptide Purity: How to Read a Chromatogram Without Overclaiming
A practical guide to interpreting peptide HPLC purity, peak area, wavelength, gradients and the questions a chromatogram cannot answer alone.
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